Purity:98% (TLC: 10%MeOH/methylene chloride; Rf = 0.33). Peptide content: 100%
Solubility:Soluble in: DMSO (25 mg/ml); Ethanol (25 mg/ml)
Storage/Stability:Store, as supplied, at -80°C for up to 2 years. May be stored at -20°C for up to 1 week. Store solutions at -80°C for up to 2 months.
Recommended Dilutions:A potent, cell permeable and selective proteasome inhibitor (Ki = 4 nM). Inhibits NF-κB activation by preventing NF-κB degradation (IC50 = 3 μM). Blocks degradation of short-lived proteins, which in turn induces HSP and ER chaperone expression, leading to thermotolerance (1 μM MG-132, 2 h.). Stimulates neurite outgrowth in PC12 cells (20 nM optimal). IC50’s for inhibition of Suc-LLVY-AMC and Z-LLL-AMC cleaving activities of proteasome were 0.85 and 0.1 μM respectively.
References:1 J.Adams and R.Stein Ann.Rep.Med.Chem. 1996 31 279