Antibody products, Antibody development, Antibody Contract Outsourcing,Antibody Services-EnoGene Biotech Co. Ltd

Custom Polyclonal Antibody Services (Mouse)

Custom Polyclonal Antibody Services (Mouse)

Starting From			To	Anti-sera	Protein A Purified IgGs
	Quantity	QC Data	Code	AS	PA
Peptide antigens from customers	~ 5 mg	>85% Pure by HPLC Correct MW by MS	MPE	Inquiry (4-12 weeks)
	~10mg				Inquiry (4-12 weeks)
Protein antigens from customers	~ 5mg	> 90% Pure and correct MW by SDS-PAGE	MPR	Inquiry (4-12 weeks)
	~10mg				Inquiry (4-12 weeks)
Peptide sequences (peptide synthesis at EnoGene)	~15mg	>85% Pure by HPLC Correct MW by MS	MPS	Inquiry (11-14 weeks)	Inquiry (11-14 weeks)
Protein sequences (Peptide design and synthesis at EnoGene)			MPD

1. MPE-AS or MPR-AS: Basic Polyclonal Antibody Package (Mouse)

Includes	Conjugation to KLH (optional) Five Balb/c mice, pre-immune bleed, four immunizations/mouse ELISA test Antigens provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
Delivery	10-20 µl of pre-immune serum ELISA results 1-2.5 ml of antisera from all mice
Time frame	Conjugation: 1 week Immunization and ELISA: 11 weeks Shipping: 1 week Total 12-13 weeks

2. MPE-PA or MPR-PA: Basic Protein A-Purified Polyclonal Antibody Package (Mouse)

Includes	Conjugation to KLH (optional) 5 Balb/c mice, pre-immune bleed, 4 immunizations/mouse Final bleed Protein A purification ELISA test Antigen provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
Delivery	10-20 µl of pre-immune serum ELISA results 2.5-12.5 mg protein A-purified antibodies Remained antigen
Time frame	Conjugation: 1 week Immunization: 10 weeks Protein A Purification and ELISA: 1-2 week Shipping: 1 week Total 13-14 weeks

3. MPS-AS or MPD-AS: Complete Peptide Polyclonal Antibody Package (Mouse)

Includes	Peptide synthesis (≤15 a.a, >85% purity, 15 mg) A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. Five Balb/c mice, pre-immune bleed, four immunizations/mouse ELISA test
Delivery	10-20 µl of pre-immune serum ELISA results 1-2.5 ml of antisera from all mice Remained unconjugated peptide, MS and HPLC report
Time frame	Peptide Synthesis: 2-3 weeks Conjugation: 1 week Immunization and ELISA: 11 weeks Shipping: 1 week Total 14-16 weeks

4. MPS-PA or MPD-PA: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)

Includes	Peptide synthesis (≤15 a.a, >85% purity, 15 mg) A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. Five Balb/C mice, pre-immune, four immunizations/mouse Antibody purification by protein A column ELISA test
Delivery	10-20 µl of pre-immune serum ELISA results 2.5-12.5 mg purified antibody from all antisera Remained unconjugated peptide, MS and HPLC report
Time frame	Peptide Synthesis: 2-3 weeks Conjugation: 1 week Immunization: 10 weeks Purification and ELISA: 1-2week Shipping: 1 week Total 15-17 weeks

Custom Polyclonal Antibody Services (Mouse)