Solubility:Soluble in DMSO (25 mg/ml warm) and ethanol (25 mg/ml warm)
Storage/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Recommended Dilutions:Potent, cell permeable and specific inhibitor of phosphatidylinositol 3- kinase (IC50 = 1.4 μM; purified PI 3-kinase). Does not significantly inhibit PKC, PKA, MAP kinase, S6 kinase, EGFrK, c-src kinase, PI 4-kinase, DAG kinase or rabbit kidney ATPase at concentrations of 50 μM. In intact neutrophils stimulated with fMet-Leu-Phe, LY 294002 completely abolished PI 3-kinase activity at 50 μM without cell toxicity. Also blocked proliferation of cultured rabbit aortic smooth muscle cells without cell death. Induces apoptosis.
References:1 C.J.Vlahos et al. J.Biol.Chem. 1994 269 5241