Brief Intro:he Annexin V-EGFP Apoptosis Detection Kit is based on the separated observation of different time of apoptosis cells. The most phosphatidylserines (PS) in cell membrane phospholipids translocate from the inner face to the outer face in the early period of apoptosis. Once PSs are on the plasma membrane surface, it can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion with annexin V, a protein that has strong natural affinity with PS. In addition, Propidium Iodide (PI) is a nucleic dye. It cannot be through cellular membrane directly, but it can pass membrane of cells in middle and late period of apoptosis and stain them red on nuclei. Detection can be analyzed by flow cytometry and fluorescence microscopy with a FITC filter. EGFP is brighter and much more photo-stable than other fluorescent reagents such as FITC, etc. KEY FEACTURE ●Easy to perform: rapid and simple procedures to perform ●High Precision: detect the cells in the early period of apoptosis, accurately differentiate and compute the ratio of normal cells, apoptosis cells and necrotic cells. High Stabilization: EGFP has high signals, hard to quench.
Experimental Methods:Annexin V-EGFP Apoptosis Detection Kit PROTOCOL