AKT1 AntibodyPDF

  • Catalog Number:

    E90003
  • Amount:

    100ul
  • Background:

    The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
  • Species:

    Rabbit
  • Isotype:

    IgG
  • Storage/Stability:

    Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Altername:

    retinoblastoma 1; RB1; OSRC; RB; p105-Rb; pRb; pp110
  • Immunogen:

    Fusion protein of human Rb
  • Purification:

    Affinity purification
  • Reactivity:

    H M
  • Applications:

    WB IHC
  • Molecular Weight:

    110kDa
  • Swiss-Prot No.:

    P06400
  • Gene ID:

    5925
  • References:

    1.Sherr, C.J. (1996) Science 274, 1672-7. 2.Nevins, J.R. (1992) Science 258, 424-9. 3.Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. 4.Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. 5.Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. 6.Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 7.Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. 8.Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. 9.Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
    WB 1:500 - 1:1000
    IHC 1:50 - 1:100
  • Price:

    225$
  • Western blot analysis of Jurkat cell and A431cell using Rb antibody.
  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

Related Products

c-Jun (Phospho-Ser63) AntibodyPDF
  • Catalog Number:

    E011001-1
  • Amount:

    50μg/50μl
  • Reactivity:

    Human, Mouse
  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
  • Swiss-Prot No.:

    P05412
  • Price:

    145.00$
c-Jun (Phospho-Ser63) AntibodyPDF
  • Catalog Number:

    E011001-2
  • Amount:

    100μg/100μl
  • Reactivity:

    Human, Mouse
  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
  • Swiss-Prot No.:

    P05412
  • Price:

    225.00$
Elk-1 (Phospho-Ser383) AntibodyPDF
  • Catalog Number:

    E011004-1
  • Amount:

    50μg/50μl
  • Reactivity:

    Human, Mouse, Rat
  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
  • Swiss-Prot No.:

    P19419
  • Price:

    145.00$
Elk-1 (Phospho-Ser383) AntibodyPDF
  • Catalog Number:

    E011004-2
  • Amount:

    100μg/100μl
  • Reactivity:

    Human, Mouse, Rat
  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
  • Swiss-Prot No.:

    P19419
  • Price:

    225.00$

Catalog Number

  • Amount:100ul
  • Price:225.00$

AbPromise