Background:Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Form of Antibody:IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:Store at -20℃/1 year. Do not aliquot the antibody.
Specificity/Sensitivity:The GAPDH antibody can detects Zebrafish endogenous GAPDH protein.
Applications:WB: 1:5,000-10,000 IHC: 1:200 IF: 1:200